WebJun 26, 2012 · After 48 h of incubation, the cells were treated with various concentrations of sirtinol and cultured for 24 or 48 h. At the end of the treatment period, 100 μ l of 1X MTT reagent were added to each well and incubated for 4 h at 37°C in the dark. WebAfter fixa- tion in Carnoy's fluid for 5 minutes, cover- slips were hydrated in ethyl alcohol series, rinsed in distilled water, and placed in Mc- Ilvaine's citric acid-disodium phosphate buffer at pH 4.0 for 10 minutes before stain- ing in 0.01% acridine orange as described by Mayor and Diwan (1961).

Acridine orange staining of a single-stranded DNA bacteriophage

WebParaffin wax Most widely used embedding medium tse processing Thymol Subs added to mayer's egg albumin after prep to prevent growth of fungus Direfringent Aside from destroying enzymes, this process will prevent putrefaction Osmic acid Fixative best for cytology smears Ziehl neelsen stain Most widely stain for demonstrating acid fast organism WebFixation is performed with vapours of a commercially available formaldehyde solution for 0.5-1 min followed by air drying and permanent mounting in DPX. After this procedure, … development antonyms in english

Fixation and permanent mounting of fluorescent probes …

WebSep 17, 2014 · The total sample of cells, both in suspension and adhered, was harvested and washed with PBS containing 1% bovine serum albumin (BSA, cat. no. A4503, Sigma-Aldrich) before fixing in 95% ice-cold ethanol containing 0.5% Tween-20 (cat. no. P9416, Sigma-Aldrich) for at least 1 h at −20°C. WebAfter fixa- tion in Carnoy's fluid for 5 minutes, cover- slips were hydrated in ethyl alcohol series, rinsed in distilled water, and placed in Mc- Ilvaine's citric acid-disodium phosphate … WebSep 25, 2012 · Actually acridine orange is useful for detection of lysosome, simultaneous experiment is needed with MDC (Mono Dansyl cadaverin ) and microscopy is … development application bundaberg